Abstract publication No. 85-23, revised in 1996) and


The research
question is clearly stated in the abstract which is: “to investigate the mechanism of
peroxynitrite-induced relaxation in the mouse aorta, the effect of
atherosclerosis on relaxation to peroxynitrite and other vasodilators, and the
effect of atherosclerosis on the expression and function of the IP3 receptor.”
abstract, p69.  These questions asked in
the article is further work on a subject the authors had previously worked on
and seems to be novel as it does not test the hypothesis with the same model or
with a different drug. However the abstract is brief and does not contain
reasons on why the questions are worth asking or how important it is that the
subject questions are answered.

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The role of IP3R in maintaining
relaxation to ONOO – in this pathology is not well understood and
the authors wanted to further examine IP3R function in atherosclerosis. There
is a sufficient body of relevant literature cited in the introduction and adds
to the context of the study. Furthermore there is background information on the
work on Peroxynitrite, IP3R and atherosclerosis. However there is no
identification of missing research to the subject that shows that findings of
the paper are novel. Finally the aims of the experiment are clearly shown at
the end of the introduction.



The methods are detailed enough which
allows for the experiment to be repeated. The suppliers of mice, IP3R,
vasodilators and other reagents are stated, this allows for ease repeatability
of the experiment. For the mice used the sex, age, weight, diet and strain were
stated in the methods. The following 
declaration “Procedures conformed to the Guide for the Care and Use of
Laboratory Animals published by the US National Institutes of Health (NIH
publication No. 85-23, revised in 1996) and Directive 2010/63/EU of the
European Parliament.” Methods, p70, shows that the experiment involved ethical
and licenced conditions in order to proceed. The termination of the mice was
recorded with the anaesthetic: sodium pentobarbital and the dose: 200 mg/mL.
There were citations in the methods but the full methods were not published
elsewhere. Group sizes are not clearly stated but are however shown in the
figure legends such as Fig 1.a. The n refers to the number of mice used for an
independent experiment with the standard error of the mean taken. The authors
did not explain the group sizes and they were not stated in the methods, they
were latter shown in figure legends to be greater than 5 however were not
stated to be equal in size. Histograms were assessed blindly by 2 independent
spectator and the staining was calculated using ImageJ software. The standard
error of the mean was calculated and n was the number of mice used for each
experiment, this allows for sufficient statistical tests to be performed by the
GraphPad system. The P value had to be